Journal:

Article Title: Anti-cytokeratin antibodies in sera of the patients with autoimmune hepatitis

doi: 10.1046/j.1365-2249.2001.01568.x

Figure Lengend Snippet: Levels of anti-human cytokeratin 8 (a), anti-cytokeratin 18 (b) or anti-cytokeratin 19 (c) antibodies in patients' sera and in sera of normal volunteers. The bar represents mean ± standard deviation.

Article Snippet: Sera diluted 1 : 100 were added to wells coated with monoclonal anti-human CK8 antibody (clone Ks 8·7, Progen Biotechnik GMBH, Heidelberg, Germany, diluted at 1 : 250) or anti-human CK18 antibody (clone 18·27, Progen Biotechnik GMBH, diluted at 1 : 250).

Techniques: Standard Deviation

Journal:

Article Title: Anti-cytokeratin antibodies in sera of the patients with autoimmune hepatitis

doi: 10.1046/j.1365-2249.2001.01568.x

Figure Lengend Snippet: Correlations between anti-CK antibodies or immune complexes in serum and other parameters in patients with autoimmune hepatitis ( n = 16)

Article Snippet: Sera diluted 1 : 100 were added to wells coated with monoclonal anti-human CK8 antibody (clone Ks 8·7, Progen Biotechnik GMBH, Heidelberg, Germany, diluted at 1 : 250) or anti-human CK18 antibody (clone 18·27, Progen Biotechnik GMBH, diluted at 1 : 250).

Techniques:

Journal:

Article Title: Anti-cytokeratin antibodies in sera of the patients with autoimmune hepatitis

doi: 10.1046/j.1365-2249.2001.01568.x

Figure Lengend Snippet: Levels of anti-human cytokeratin 8 (a), anti-cytokeratin 18 (b), or anti-cytokeratin 19 (c) antibodies in AIH patients' sera before and after treatment. The bar represents mean ± standard deviation. *P < 0.005; **P < 0.01.

Article Snippet: Sera diluted 1 : 100 were added to wells coated with monoclonal anti-human CK8 antibody (clone Ks 8·7, Progen Biotechnik GMBH, Heidelberg, Germany, diluted at 1 : 250) or anti-human CK18 antibody (clone 18·27, Progen Biotechnik GMBH, diluted at 1 : 250).

Techniques: Standard Deviation

Journal:

Article Title: Anti-cytokeratin antibodies in sera of the patients with autoimmune hepatitis

doi: 10.1046/j.1365-2249.2001.01568.x

Figure Lengend Snippet: Levels of cytokeratin 8:anti-cytokeratin 8 antibody immune complexes (a) or cytokeratin 18:anti-cytokeratin 18 antibody immune complexes (b) in AIH patients' sera (n = 16) and in sera of normal volunteers (n = 12) and in sera of patients with CH‐C (n = 19). The bar represents mean ± standard deviation. *P < 0.05; **P < 0.01.

Article Snippet: Sera diluted 1 : 100 were added to wells coated with monoclonal anti-human CK8 antibody (clone Ks 8·7, Progen Biotechnik GMBH, Heidelberg, Germany, diluted at 1 : 250) or anti-human CK18 antibody (clone 18·27, Progen Biotechnik GMBH, diluted at 1 : 250).

Techniques: Standard Deviation

Journal:

Article Title: Anti-cytokeratin antibodies in sera of the patients with autoimmune hepatitis

doi: 10.1046/j.1365-2249.2001.01568.x

Figure Lengend Snippet: Immunohistochemical staining of the liver with anti-human cytokeratin 8 (a,b), anti-cytokeratin 18 (c,d), or anti-cytokeratin 19 (e,f) monoclonal antibodies in patients with autoimmune hepatitis (AIH). Although some hepatocytes in AIH are stained strongly by anti-cytokeratin 8 monoclonal antibody, other hepatocytes are not stained (a). Hepatocytes positive for cytokeratin 8 seem to reveal a mosaic pattern. In contrast, hepatocytes obtained from patients with chronic active hepatitis are diffusely stained for cytokeratin 8 (b). Although some hepatocytes in AIH are stained strongly by anti-cytokeratin 18 monoclonal antibody, other intermingled hepatocytes are not stained (c). In contrast, hepatocytes obtained from patients with chronic active hepatitis are diffusely stained for cytokeratin 18 (d). Immunohistochemical staining of the liver with anti-human cytokeratin 19 monoclonal antibody in patients with AIH demonstrates the strong positive reaction in the cytoplasm of bile duct cells (e). Some periportal hepatocytes in patients with AIH are also stained positively by anti-CK19 monoclonal antibody (e). In contrast, hepatocytes obtained from 12 patients with chronic active hepatitis are completely negative for cytokeratin 19 (f). In addition, staining intensity of bile duct in patients with AIH is much stronger than that of patients with chronic active hepatitis (compare e and f). Avidin–biotin–peroxidase complex method; Magnification a,b,e,f: ×220, c,d: ×350.

Article Snippet: Sera diluted 1 : 100 were added to wells coated with monoclonal anti-human CK8 antibody (clone Ks 8·7, Progen Biotechnik GMBH, Heidelberg, Germany, diluted at 1 : 250) or anti-human CK18 antibody (clone 18·27, Progen Biotechnik GMBH, diluted at 1 : 250).

Techniques: Immunohistochemical staining, Staining, Bioprocessing, Avidin-Biotin Assay

Journal: The Journal of Cell Biology

Article Title: Mice with targeted disruption of the fatty acid transport protein 4 (Fatp 4, Slc27a4) gene show features of lethal restrictive dermopathy

doi: 10.1083/jcb.200207080

Figure Lengend Snippet: Differentiation of neonatal skin of newborn Fatp4 −/− and control Fatp4 +/+ mice, as shown by immunofluorescence microscopy. (A) Immunofluorescence microscopy of keratin K14 in dorsal skin. In the Fatp4 +/+ control mice, K14 is restricted to the stratum basale (sb) and in the Fatp4 −/− mice is additionally detected in the first layers of the stratum spinosum (open arrow). (B) With keratin K10 antibodies, the stratum spinosum and the stratum granulosum (ss+sg) are strongly stained in both wild-type and Fatp4 −/− mice. However, note that both layers are thicker in the mutant. (C) Immunostaining of K6 is negative in the Fatp4 +/+, but intense in the upper suprabasal layers, including the stratum corneum (sc), of the Fatp4 −/− mice. (D) Loricrin as a typical protein of the cornified envelope is detected in the stratum granulosum (sg) and seems to be a bit more prominent in the mutant mice. (E) The presence of occludin in the plasma membranes of the stratum granulosum (bracket; sb, stratum basale) is seen in a somewhat broader appearing zone in the mutant mice, indicating an intact tight junction barrier in both kinds of mice. To the right of each of the immunofluorescence micrographs are the specific phase-contrast images of the same sections. Blue: DAPI nuclear staining. Bars, 50 μm.

Article Snippet: For comparison, antibodies used in immunohistochemistry were as follows: (1) rabbit antibodies to occludin, claudin 1, protein ZO-1 (all from Zytomed), to keratins K16 (gift of Dr. P. Coulombe, Johns Hopkins University School of Medicine, Baltimore, MD) and K10 (gift of Dr. D. Roop, Baylor College, Houston, TX), to periplakin and loricrin (both antisera were gifts of Dr. D. Hohl, Hopital de Beaumont, Lausanne, Switzerland); (2) guinea pig antibodies against keratins K2e, K5 and K14 (all from Progen Biotechnik); and (3) mouse antibodies to keratin K6 (clone KA12; Progen Biotechnik), filaggrin (mouse ascites; from Quartett Co., or from Paesel & Lorei) and to transglutaminase 1, TG-1 (Cell Systems).

Techniques: Control, Immunofluorescence, Microscopy, Staining, Mutagenesis, Immunostaining, Clinical Proteomics